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bright blue cathode running buffer  (Thermo Fisher)


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    Thermo Fisher bright blue cathode running buffer
    Bright Blue Cathode Running Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bright+blue+cathode+running+buffer/light+blue+cathode+buffer/pm40398393-601-136-141
    Average 90 stars, based on 1 article reviews
    bright blue cathode running buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Recombinant:

    Article Title: Targeting mitochondrial complex I of CD177 + neutrophils alleviates lung ischemia-reperfusion injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human CD177 Antibody Biolegend Cat#315806; RRID:AB_2564281 PE/Cyanine7 anti-human CD45 Antibody Biolegend Cat#368532; RRID:AB_2715892 FITC anti-human CD66b Antibody Biolegend Cat#305104; RRID:AB_314496 FITC anti-mouse Ly-6G Antibody Biolegend Cat#127606; RRID:AB_1236494 PE/Cyanine7 anti-mouse/human CD11b Antibody Biolegend Cat#101216; RRID:AB_312799 PerCP-Cyanine5.5 anti-mouse/human CD45 Antibody ThermoFisher Cat#45-0451-82; RRID:AB_1107002 Rabbit polyclonal anti-Histone H3 (citrulline R2 + R8 + R17) Abcam Cat#ab281584 Rat monoclonal anti-Ly-6G BD biosciences Cat#551459; RRID:AB_394206 Mouse monoclonal anti-Histone H1 Abcam Cat#ab218417; RRID:AB_218417 Rabbit monoclonal anti-CD177 Santa Cruz Biotechnology Cat# sc-376329; RRID:AB_10988032 Rabbit polyclonal anti-CD177 Abclonal Cat#A6550; RRID:AB_2767144 Rat monoclonal anti-CD11b Abcam Cat#ab8878; RRID:AB_306831 Rabbit monoclonal anti-CD11b Abcam Cat# ab52478; RRID:AB_868788 Rabbit monoclonal anti-CD11b Abcam Cat#ab184308; RRID:AB_2889154 Mouse monoclonal anti-ILK Protientech Cat#67724-1-Ig; RRID:AB_2882910 Rabbit monoclonal anti-ILK Abcam Cat#ab52480; RRID:AB_881194 Rabbit monoclonal anti-CD11b Abcam Cat#ab133357; RRID:AB_2650514 Rabbit monoclonal anti-AKT Abcam Cat#ab179463; RRID:AB_2810977 Rabbit monoclonal anti-phospho-AKT Abcam Cat#ab192623; RRID:AB_2934087 Rabbit monoclonal anti-mTOR Abcam Cat#ab134903; RRID:AB_2800465 Rabbit monoclonal anti-phospho-mTOR Abcam Cat#ab109268; RRID:AB_10888105 Rabbit polyclonal anti- ATP5A1 Protientech Cat#14676-1-AP; RRID:AB_2061761 Rabbit polyclonal anti-UQCRC2 Protientech Cat#14742-1-AP; RRID:AB_2241442 (Continued on next page) Cell Reports Medicine 6, 102140, May 20, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-SDHA Protientech Cat#14865-1-AP; RRID:AB_11182164 Rabbit polyclonal anti-NDUFS1 Protientech Cat#12444-1-AP; RRID:AB_2282657 Rabbit polyclonal anti-COXIV Protientech Cat#11242-1-AP; RRID:AB_2085278 Rabbit polyclonal anti-GAPDH Servicebio Cat# GB11002-100; RRID:AB_2904017 Rabbit polyclonal anti-β-actin Biolegend Cat#622102; RRID:AB_315946 Biological samples Peripheral blood Shanghai Pulmonary Hospital N/A Chemicals, peptides, and recombinant proteins IACS-010759 MCE Cat#HY-112037 MHY1485 MCE Cat#HY-B0795 AZD8055 MCE Cat#HY-10422 SC-79 MCE Cat#HY-18749 Ipatasertib MCE Cat#HY-15186 QLT0267 Cayman Chemical Cat#866409-68-9 Celsior solution Genzyme Corp NA PolymorphPrep Axis Shield Cat#1114683 Erythrocytes lysate Biosharp Cat#BL503B Human BD Fc Block BD biosciences Cat#564220 Mouse BD Fc Block BD biosciences Cat#553141 FACS buffer BD biosciences Cat#554656 LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit ThermoFisher Cat# L34973 TRIzol Invitrogen Cat#15596018CN Sytox green Alphabio Cat#AP6352 RIPA buffer Beyotime Cat#P0013C PMSF and phosphatase inhibitors Beyotime Cat#P1045 Digitonin Invitrogen Cat#BN2008 Coomassie blue G250 Invitrogen Cat#BN2008 Dark blue cathode running buffer Invitrogen Cat#BN2002 Bright blue cathode running buffer Invitrogen CatBN2007 Electron microscope fixative Servicebio Cat#G1102 MitoSOXTM Deep Red Dojindo Cat#MT14 MitoTrackerTM Red CMXRos Beyotime Cat#C1049B MACS Tissue Storage Solution Miltenyi Cat#130-100-008 Collagenase Type I Thermo Fisher Cat#17100017 Dispase Roche Cat#04942086001 DNase Roche Cat#10104159001 Critical commercial assays Mouse Bone Marrow Neutrophil Isolation Kit Solarbio Cat#P8550 Human Myeloperoxidase ELISA Kit Abcam Cat#ab119605 Mouse Myeloperoxidase ELISA Kit Abcam Cat#ab275109 Mouse Direct PCR Kit Selleck B40013 QubitTM dsDNA Quantification Kit Thermo Fisher Cat#Q32851 Reactive Oxygen Species Assay Kit Servicebio Cat#G1706-100T PrimeScript RT reagent kit TaKaRa Cat#RR037A (Continued on next page) e2 Cell Reports Medicine 6, 102140, May 20, 2025 ..

    Blocking Assay:

    Article Title: Targeting mitochondrial complex I of CD177 + neutrophils alleviates lung ischemia-reperfusion injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human CD177 Antibody Biolegend Cat#315806; RRID:AB_2564281 PE/Cyanine7 anti-human CD45 Antibody Biolegend Cat#368532; RRID:AB_2715892 FITC anti-human CD66b Antibody Biolegend Cat#305104; RRID:AB_314496 FITC anti-mouse Ly-6G Antibody Biolegend Cat#127606; RRID:AB_1236494 PE/Cyanine7 anti-mouse/human CD11b Antibody Biolegend Cat#101216; RRID:AB_312799 PerCP-Cyanine5.5 anti-mouse/human CD45 Antibody ThermoFisher Cat#45-0451-82; RRID:AB_1107002 Rabbit polyclonal anti-Histone H3 (citrulline R2 + R8 + R17) Abcam Cat#ab281584 Rat monoclonal anti-Ly-6G BD biosciences Cat#551459; RRID:AB_394206 Mouse monoclonal anti-Histone H1 Abcam Cat#ab218417; RRID:AB_218417 Rabbit monoclonal anti-CD177 Santa Cruz Biotechnology Cat# sc-376329; RRID:AB_10988032 Rabbit polyclonal anti-CD177 Abclonal Cat#A6550; RRID:AB_2767144 Rat monoclonal anti-CD11b Abcam Cat#ab8878; RRID:AB_306831 Rabbit monoclonal anti-CD11b Abcam Cat# ab52478; RRID:AB_868788 Rabbit monoclonal anti-CD11b Abcam Cat#ab184308; RRID:AB_2889154 Mouse monoclonal anti-ILK Protientech Cat#67724-1-Ig; RRID:AB_2882910 Rabbit monoclonal anti-ILK Abcam Cat#ab52480; RRID:AB_881194 Rabbit monoclonal anti-CD11b Abcam Cat#ab133357; RRID:AB_2650514 Rabbit monoclonal anti-AKT Abcam Cat#ab179463; RRID:AB_2810977 Rabbit monoclonal anti-phospho-AKT Abcam Cat#ab192623; RRID:AB_2934087 Rabbit monoclonal anti-mTOR Abcam Cat#ab134903; RRID:AB_2800465 Rabbit monoclonal anti-phospho-mTOR Abcam Cat#ab109268; RRID:AB_10888105 Rabbit polyclonal anti- ATP5A1 Protientech Cat#14676-1-AP; RRID:AB_2061761 Rabbit polyclonal anti-UQCRC2 Protientech Cat#14742-1-AP; RRID:AB_2241442 (Continued on next page) Cell Reports Medicine 6, 102140, May 20, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-SDHA Protientech Cat#14865-1-AP; RRID:AB_11182164 Rabbit polyclonal anti-NDUFS1 Protientech Cat#12444-1-AP; RRID:AB_2282657 Rabbit polyclonal anti-COXIV Protientech Cat#11242-1-AP; RRID:AB_2085278 Rabbit polyclonal anti-GAPDH Servicebio Cat# GB11002-100; RRID:AB_2904017 Rabbit polyclonal anti-β-actin Biolegend Cat#622102; RRID:AB_315946 Biological samples Peripheral blood Shanghai Pulmonary Hospital N/A Chemicals, peptides, and recombinant proteins IACS-010759 MCE Cat#HY-112037 MHY1485 MCE Cat#HY-B0795 AZD8055 MCE Cat#HY-10422 SC-79 MCE Cat#HY-18749 Ipatasertib MCE Cat#HY-15186 QLT0267 Cayman Chemical Cat#866409-68-9 Celsior solution Genzyme Corp NA PolymorphPrep Axis Shield Cat#1114683 Erythrocytes lysate Biosharp Cat#BL503B Human BD Fc Block BD biosciences Cat#564220 Mouse BD Fc Block BD biosciences Cat#553141 FACS buffer BD biosciences Cat#554656 LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit ThermoFisher Cat# L34973 TRIzol Invitrogen Cat#15596018CN Sytox green Alphabio Cat#AP6352 RIPA buffer Beyotime Cat#P0013C PMSF and phosphatase inhibitors Beyotime Cat#P1045 Digitonin Invitrogen Cat#BN2008 Coomassie blue G250 Invitrogen Cat#BN2008 Dark blue cathode running buffer Invitrogen Cat#BN2002 Bright blue cathode running buffer Invitrogen CatBN2007 Electron microscope fixative Servicebio Cat#G1102 MitoSOXTM Deep Red Dojindo Cat#MT14 MitoTrackerTM Red CMXRos Beyotime Cat#C1049B MACS Tissue Storage Solution Miltenyi Cat#130-100-008 Collagenase Type I Thermo Fisher Cat#17100017 Dispase Roche Cat#04942086001 DNase Roche Cat#10104159001 Critical commercial assays Mouse Bone Marrow Neutrophil Isolation Kit Solarbio Cat#P8550 Human Myeloperoxidase ELISA Kit Abcam Cat#ab119605 Mouse Myeloperoxidase ELISA Kit Abcam Cat#ab275109 Mouse Direct PCR Kit Selleck B40013 QubitTM dsDNA Quantification Kit Thermo Fisher Cat#Q32851 Reactive Oxygen Species Assay Kit Servicebio Cat#G1706-100T PrimeScript RT reagent kit TaKaRa Cat#RR037A (Continued on next page) e2 Cell Reports Medicine 6, 102140, May 20, 2025 ..

    FACS:

    Article Title: Targeting mitochondrial complex I of CD177 + neutrophils alleviates lung ischemia-reperfusion injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human CD177 Antibody Biolegend Cat#315806; RRID:AB_2564281 PE/Cyanine7 anti-human CD45 Antibody Biolegend Cat#368532; RRID:AB_2715892 FITC anti-human CD66b Antibody Biolegend Cat#305104; RRID:AB_314496 FITC anti-mouse Ly-6G Antibody Biolegend Cat#127606; RRID:AB_1236494 PE/Cyanine7 anti-mouse/human CD11b Antibody Biolegend Cat#101216; RRID:AB_312799 PerCP-Cyanine5.5 anti-mouse/human CD45 Antibody ThermoFisher Cat#45-0451-82; RRID:AB_1107002 Rabbit polyclonal anti-Histone H3 (citrulline R2 + R8 + R17) Abcam Cat#ab281584 Rat monoclonal anti-Ly-6G BD biosciences Cat#551459; RRID:AB_394206 Mouse monoclonal anti-Histone H1 Abcam Cat#ab218417; RRID:AB_218417 Rabbit monoclonal anti-CD177 Santa Cruz Biotechnology Cat# sc-376329; RRID:AB_10988032 Rabbit polyclonal anti-CD177 Abclonal Cat#A6550; RRID:AB_2767144 Rat monoclonal anti-CD11b Abcam Cat#ab8878; RRID:AB_306831 Rabbit monoclonal anti-CD11b Abcam Cat# ab52478; RRID:AB_868788 Rabbit monoclonal anti-CD11b Abcam Cat#ab184308; RRID:AB_2889154 Mouse monoclonal anti-ILK Protientech Cat#67724-1-Ig; RRID:AB_2882910 Rabbit monoclonal anti-ILK Abcam Cat#ab52480; RRID:AB_881194 Rabbit monoclonal anti-CD11b Abcam Cat#ab133357; RRID:AB_2650514 Rabbit monoclonal anti-AKT Abcam Cat#ab179463; RRID:AB_2810977 Rabbit monoclonal anti-phospho-AKT Abcam Cat#ab192623; RRID:AB_2934087 Rabbit monoclonal anti-mTOR Abcam Cat#ab134903; RRID:AB_2800465 Rabbit monoclonal anti-phospho-mTOR Abcam Cat#ab109268; RRID:AB_10888105 Rabbit polyclonal anti- ATP5A1 Protientech Cat#14676-1-AP; RRID:AB_2061761 Rabbit polyclonal anti-UQCRC2 Protientech Cat#14742-1-AP; RRID:AB_2241442 (Continued on next page) Cell Reports Medicine 6, 102140, May 20, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-SDHA Protientech Cat#14865-1-AP; RRID:AB_11182164 Rabbit polyclonal anti-NDUFS1 Protientech Cat#12444-1-AP; RRID:AB_2282657 Rabbit polyclonal anti-COXIV Protientech Cat#11242-1-AP; RRID:AB_2085278 Rabbit polyclonal anti-GAPDH Servicebio Cat# GB11002-100; RRID:AB_2904017 Rabbit polyclonal anti-β-actin Biolegend Cat#622102; RRID:AB_315946 Biological samples Peripheral blood Shanghai Pulmonary Hospital N/A Chemicals, peptides, and recombinant proteins IACS-010759 MCE Cat#HY-112037 MHY1485 MCE Cat#HY-B0795 AZD8055 MCE Cat#HY-10422 SC-79 MCE Cat#HY-18749 Ipatasertib MCE Cat#HY-15186 QLT0267 Cayman Chemical Cat#866409-68-9 Celsior solution Genzyme Corp NA PolymorphPrep Axis Shield Cat#1114683 Erythrocytes lysate Biosharp Cat#BL503B Human BD Fc Block BD biosciences Cat#564220 Mouse BD Fc Block BD biosciences Cat#553141 FACS buffer BD biosciences Cat#554656 LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit ThermoFisher Cat# L34973 TRIzol Invitrogen Cat#15596018CN Sytox green Alphabio Cat#AP6352 RIPA buffer Beyotime Cat#P0013C PMSF and phosphatase inhibitors Beyotime Cat#P1045 Digitonin Invitrogen Cat#BN2008 Coomassie blue G250 Invitrogen Cat#BN2008 Dark blue cathode running buffer Invitrogen Cat#BN2002 Bright blue cathode running buffer Invitrogen CatBN2007 Electron microscope fixative Servicebio Cat#G1102 MitoSOXTM Deep Red Dojindo Cat#MT14 MitoTrackerTM Red CMXRos Beyotime Cat#C1049B MACS Tissue Storage Solution Miltenyi Cat#130-100-008 Collagenase Type I Thermo Fisher Cat#17100017 Dispase Roche Cat#04942086001 DNase Roche Cat#10104159001 Critical commercial assays Mouse Bone Marrow Neutrophil Isolation Kit Solarbio Cat#P8550 Human Myeloperoxidase ELISA Kit Abcam Cat#ab119605 Mouse Myeloperoxidase ELISA Kit Abcam Cat#ab275109 Mouse Direct PCR Kit Selleck B40013 QubitTM dsDNA Quantification Kit Thermo Fisher Cat#Q32851 Reactive Oxygen Species Assay Kit Servicebio Cat#G1706-100T PrimeScript RT reagent kit TaKaRa Cat#RR037A (Continued on next page) e2 Cell Reports Medicine 6, 102140, May 20, 2025 ..

    Staining:

    Article Title: Targeting mitochondrial complex I of CD177 + neutrophils alleviates lung ischemia-reperfusion injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human CD177 Antibody Biolegend Cat#315806; RRID:AB_2564281 PE/Cyanine7 anti-human CD45 Antibody Biolegend Cat#368532; RRID:AB_2715892 FITC anti-human CD66b Antibody Biolegend Cat#305104; RRID:AB_314496 FITC anti-mouse Ly-6G Antibody Biolegend Cat#127606; RRID:AB_1236494 PE/Cyanine7 anti-mouse/human CD11b Antibody Biolegend Cat#101216; RRID:AB_312799 PerCP-Cyanine5.5 anti-mouse/human CD45 Antibody ThermoFisher Cat#45-0451-82; RRID:AB_1107002 Rabbit polyclonal anti-Histone H3 (citrulline R2 + R8 + R17) Abcam Cat#ab281584 Rat monoclonal anti-Ly-6G BD biosciences Cat#551459; RRID:AB_394206 Mouse monoclonal anti-Histone H1 Abcam Cat#ab218417; RRID:AB_218417 Rabbit monoclonal anti-CD177 Santa Cruz Biotechnology Cat# sc-376329; RRID:AB_10988032 Rabbit polyclonal anti-CD177 Abclonal Cat#A6550; RRID:AB_2767144 Rat monoclonal anti-CD11b Abcam Cat#ab8878; RRID:AB_306831 Rabbit monoclonal anti-CD11b Abcam Cat# ab52478; RRID:AB_868788 Rabbit monoclonal anti-CD11b Abcam Cat#ab184308; RRID:AB_2889154 Mouse monoclonal anti-ILK Protientech Cat#67724-1-Ig; RRID:AB_2882910 Rabbit monoclonal anti-ILK Abcam Cat#ab52480; RRID:AB_881194 Rabbit monoclonal anti-CD11b Abcam Cat#ab133357; RRID:AB_2650514 Rabbit monoclonal anti-AKT Abcam Cat#ab179463; RRID:AB_2810977 Rabbit monoclonal anti-phospho-AKT Abcam Cat#ab192623; RRID:AB_2934087 Rabbit monoclonal anti-mTOR Abcam Cat#ab134903; RRID:AB_2800465 Rabbit monoclonal anti-phospho-mTOR Abcam Cat#ab109268; RRID:AB_10888105 Rabbit polyclonal anti- ATP5A1 Protientech Cat#14676-1-AP; RRID:AB_2061761 Rabbit polyclonal anti-UQCRC2 Protientech Cat#14742-1-AP; RRID:AB_2241442 (Continued on next page) Cell Reports Medicine 6, 102140, May 20, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-SDHA Protientech Cat#14865-1-AP; RRID:AB_11182164 Rabbit polyclonal anti-NDUFS1 Protientech Cat#12444-1-AP; RRID:AB_2282657 Rabbit polyclonal anti-COXIV Protientech Cat#11242-1-AP; RRID:AB_2085278 Rabbit polyclonal anti-GAPDH Servicebio Cat# GB11002-100; RRID:AB_2904017 Rabbit polyclonal anti-β-actin Biolegend Cat#622102; RRID:AB_315946 Biological samples Peripheral blood Shanghai Pulmonary Hospital N/A Chemicals, peptides, and recombinant proteins IACS-010759 MCE Cat#HY-112037 MHY1485 MCE Cat#HY-B0795 AZD8055 MCE Cat#HY-10422 SC-79 MCE Cat#HY-18749 Ipatasertib MCE Cat#HY-15186 QLT0267 Cayman Chemical Cat#866409-68-9 Celsior solution Genzyme Corp NA PolymorphPrep Axis Shield Cat#1114683 Erythrocytes lysate Biosharp Cat#BL503B Human BD Fc Block BD biosciences Cat#564220 Mouse BD Fc Block BD biosciences Cat#553141 FACS buffer BD biosciences Cat#554656 LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit ThermoFisher Cat# L34973 TRIzol Invitrogen Cat#15596018CN Sytox green Alphabio Cat#AP6352 RIPA buffer Beyotime Cat#P0013C PMSF and phosphatase inhibitors Beyotime Cat#P1045 Digitonin Invitrogen Cat#BN2008 Coomassie blue G250 Invitrogen Cat#BN2008 Dark blue cathode running buffer Invitrogen Cat#BN2002 Bright blue cathode running buffer Invitrogen CatBN2007 Electron microscope fixative Servicebio Cat#G1102 MitoSOXTM Deep Red Dojindo Cat#MT14 MitoTrackerTM Red CMXRos Beyotime Cat#C1049B MACS Tissue Storage Solution Miltenyi Cat#130-100-008 Collagenase Type I Thermo Fisher Cat#17100017 Dispase Roche Cat#04942086001 DNase Roche Cat#10104159001 Critical commercial assays Mouse Bone Marrow Neutrophil Isolation Kit Solarbio Cat#P8550 Human Myeloperoxidase ELISA Kit Abcam Cat#ab119605 Mouse Myeloperoxidase ELISA Kit Abcam Cat#ab275109 Mouse Direct PCR Kit Selleck B40013 QubitTM dsDNA Quantification Kit Thermo Fisher Cat#Q32851 Reactive Oxygen Species Assay Kit Servicebio Cat#G1706-100T PrimeScript RT reagent kit TaKaRa Cat#RR037A (Continued on next page) e2 Cell Reports Medicine 6, 102140, May 20, 2025 ..

    Microscopy:

    Article Title: Targeting mitochondrial complex I of CD177 + neutrophils alleviates lung ischemia-reperfusion injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human CD177 Antibody Biolegend Cat#315806; RRID:AB_2564281 PE/Cyanine7 anti-human CD45 Antibody Biolegend Cat#368532; RRID:AB_2715892 FITC anti-human CD66b Antibody Biolegend Cat#305104; RRID:AB_314496 FITC anti-mouse Ly-6G Antibody Biolegend Cat#127606; RRID:AB_1236494 PE/Cyanine7 anti-mouse/human CD11b Antibody Biolegend Cat#101216; RRID:AB_312799 PerCP-Cyanine5.5 anti-mouse/human CD45 Antibody ThermoFisher Cat#45-0451-82; RRID:AB_1107002 Rabbit polyclonal anti-Histone H3 (citrulline R2 + R8 + R17) Abcam Cat#ab281584 Rat monoclonal anti-Ly-6G BD biosciences Cat#551459; RRID:AB_394206 Mouse monoclonal anti-Histone H1 Abcam Cat#ab218417; RRID:AB_218417 Rabbit monoclonal anti-CD177 Santa Cruz Biotechnology Cat# sc-376329; RRID:AB_10988032 Rabbit polyclonal anti-CD177 Abclonal Cat#A6550; RRID:AB_2767144 Rat monoclonal anti-CD11b Abcam Cat#ab8878; RRID:AB_306831 Rabbit monoclonal anti-CD11b Abcam Cat# ab52478; RRID:AB_868788 Rabbit monoclonal anti-CD11b Abcam Cat#ab184308; RRID:AB_2889154 Mouse monoclonal anti-ILK Protientech Cat#67724-1-Ig; RRID:AB_2882910 Rabbit monoclonal anti-ILK Abcam Cat#ab52480; RRID:AB_881194 Rabbit monoclonal anti-CD11b Abcam Cat#ab133357; RRID:AB_2650514 Rabbit monoclonal anti-AKT Abcam Cat#ab179463; RRID:AB_2810977 Rabbit monoclonal anti-phospho-AKT Abcam Cat#ab192623; RRID:AB_2934087 Rabbit monoclonal anti-mTOR Abcam Cat#ab134903; RRID:AB_2800465 Rabbit monoclonal anti-phospho-mTOR Abcam Cat#ab109268; RRID:AB_10888105 Rabbit polyclonal anti- ATP5A1 Protientech Cat#14676-1-AP; RRID:AB_2061761 Rabbit polyclonal anti-UQCRC2 Protientech Cat#14742-1-AP; RRID:AB_2241442 (Continued on next page) Cell Reports Medicine 6, 102140, May 20, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-SDHA Protientech Cat#14865-1-AP; RRID:AB_11182164 Rabbit polyclonal anti-NDUFS1 Protientech Cat#12444-1-AP; RRID:AB_2282657 Rabbit polyclonal anti-COXIV Protientech Cat#11242-1-AP; RRID:AB_2085278 Rabbit polyclonal anti-GAPDH Servicebio Cat# GB11002-100; RRID:AB_2904017 Rabbit polyclonal anti-β-actin Biolegend Cat#622102; RRID:AB_315946 Biological samples Peripheral blood Shanghai Pulmonary Hospital N/A Chemicals, peptides, and recombinant proteins IACS-010759 MCE Cat#HY-112037 MHY1485 MCE Cat#HY-B0795 AZD8055 MCE Cat#HY-10422 SC-79 MCE Cat#HY-18749 Ipatasertib MCE Cat#HY-15186 QLT0267 Cayman Chemical Cat#866409-68-9 Celsior solution Genzyme Corp NA PolymorphPrep Axis Shield Cat#1114683 Erythrocytes lysate Biosharp Cat#BL503B Human BD Fc Block BD biosciences Cat#564220 Mouse BD Fc Block BD biosciences Cat#553141 FACS buffer BD biosciences Cat#554656 LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit ThermoFisher Cat# L34973 TRIzol Invitrogen Cat#15596018CN Sytox green Alphabio Cat#AP6352 RIPA buffer Beyotime Cat#P0013C PMSF and phosphatase inhibitors Beyotime Cat#P1045 Digitonin Invitrogen Cat#BN2008 Coomassie blue G250 Invitrogen Cat#BN2008 Dark blue cathode running buffer Invitrogen Cat#BN2002 Bright blue cathode running buffer Invitrogen CatBN2007 Electron microscope fixative Servicebio Cat#G1102 MitoSOXTM Deep Red Dojindo Cat#MT14 MitoTrackerTM Red CMXRos Beyotime Cat#C1049B MACS Tissue Storage Solution Miltenyi Cat#130-100-008 Collagenase Type I Thermo Fisher Cat#17100017 Dispase Roche Cat#04942086001 DNase Roche Cat#10104159001 Critical commercial assays Mouse Bone Marrow Neutrophil Isolation Kit Solarbio Cat#P8550 Human Myeloperoxidase ELISA Kit Abcam Cat#ab119605 Mouse Myeloperoxidase ELISA Kit Abcam Cat#ab275109 Mouse Direct PCR Kit Selleck B40013 QubitTM dsDNA Quantification Kit Thermo Fisher Cat#Q32851 Reactive Oxygen Species Assay Kit Servicebio Cat#G1706-100T PrimeScript RT reagent kit TaKaRa Cat#RR037A (Continued on next page) e2 Cell Reports Medicine 6, 102140, May 20, 2025 ..

    Magnetic Cell Separation:

    Article Title: Targeting mitochondrial complex I of CD177 + neutrophils alleviates lung ischemia-reperfusion injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human CD177 Antibody Biolegend Cat#315806; RRID:AB_2564281 PE/Cyanine7 anti-human CD45 Antibody Biolegend Cat#368532; RRID:AB_2715892 FITC anti-human CD66b Antibody Biolegend Cat#305104; RRID:AB_314496 FITC anti-mouse Ly-6G Antibody Biolegend Cat#127606; RRID:AB_1236494 PE/Cyanine7 anti-mouse/human CD11b Antibody Biolegend Cat#101216; RRID:AB_312799 PerCP-Cyanine5.5 anti-mouse/human CD45 Antibody ThermoFisher Cat#45-0451-82; RRID:AB_1107002 Rabbit polyclonal anti-Histone H3 (citrulline R2 + R8 + R17) Abcam Cat#ab281584 Rat monoclonal anti-Ly-6G BD biosciences Cat#551459; RRID:AB_394206 Mouse monoclonal anti-Histone H1 Abcam Cat#ab218417; RRID:AB_218417 Rabbit monoclonal anti-CD177 Santa Cruz Biotechnology Cat# sc-376329; RRID:AB_10988032 Rabbit polyclonal anti-CD177 Abclonal Cat#A6550; RRID:AB_2767144 Rat monoclonal anti-CD11b Abcam Cat#ab8878; RRID:AB_306831 Rabbit monoclonal anti-CD11b Abcam Cat# ab52478; RRID:AB_868788 Rabbit monoclonal anti-CD11b Abcam Cat#ab184308; RRID:AB_2889154 Mouse monoclonal anti-ILK Protientech Cat#67724-1-Ig; RRID:AB_2882910 Rabbit monoclonal anti-ILK Abcam Cat#ab52480; RRID:AB_881194 Rabbit monoclonal anti-CD11b Abcam Cat#ab133357; RRID:AB_2650514 Rabbit monoclonal anti-AKT Abcam Cat#ab179463; RRID:AB_2810977 Rabbit monoclonal anti-phospho-AKT Abcam Cat#ab192623; RRID:AB_2934087 Rabbit monoclonal anti-mTOR Abcam Cat#ab134903; RRID:AB_2800465 Rabbit monoclonal anti-phospho-mTOR Abcam Cat#ab109268; RRID:AB_10888105 Rabbit polyclonal anti- ATP5A1 Protientech Cat#14676-1-AP; RRID:AB_2061761 Rabbit polyclonal anti-UQCRC2 Protientech Cat#14742-1-AP; RRID:AB_2241442 (Continued on next page) Cell Reports Medicine 6, 102140, May 20, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-SDHA Protientech Cat#14865-1-AP; RRID:AB_11182164 Rabbit polyclonal anti-NDUFS1 Protientech Cat#12444-1-AP; RRID:AB_2282657 Rabbit polyclonal anti-COXIV Protientech Cat#11242-1-AP; RRID:AB_2085278 Rabbit polyclonal anti-GAPDH Servicebio Cat# GB11002-100; RRID:AB_2904017 Rabbit polyclonal anti-β-actin Biolegend Cat#622102; RRID:AB_315946 Biological samples Peripheral blood Shanghai Pulmonary Hospital N/A Chemicals, peptides, and recombinant proteins IACS-010759 MCE Cat#HY-112037 MHY1485 MCE Cat#HY-B0795 AZD8055 MCE Cat#HY-10422 SC-79 MCE Cat#HY-18749 Ipatasertib MCE Cat#HY-15186 QLT0267 Cayman Chemical Cat#866409-68-9 Celsior solution Genzyme Corp NA PolymorphPrep Axis Shield Cat#1114683 Erythrocytes lysate Biosharp Cat#BL503B Human BD Fc Block BD biosciences Cat#564220 Mouse BD Fc Block BD biosciences Cat#553141 FACS buffer BD biosciences Cat#554656 LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit ThermoFisher Cat# L34973 TRIzol Invitrogen Cat#15596018CN Sytox green Alphabio Cat#AP6352 RIPA buffer Beyotime Cat#P0013C PMSF and phosphatase inhibitors Beyotime Cat#P1045 Digitonin Invitrogen Cat#BN2008 Coomassie blue G250 Invitrogen Cat#BN2008 Dark blue cathode running buffer Invitrogen Cat#BN2002 Bright blue cathode running buffer Invitrogen CatBN2007 Electron microscope fixative Servicebio Cat#G1102 MitoSOXTM Deep Red Dojindo Cat#MT14 MitoTrackerTM Red CMXRos Beyotime Cat#C1049B MACS Tissue Storage Solution Miltenyi Cat#130-100-008 Collagenase Type I Thermo Fisher Cat#17100017 Dispase Roche Cat#04942086001 DNase Roche Cat#10104159001 Critical commercial assays Mouse Bone Marrow Neutrophil Isolation Kit Solarbio Cat#P8550 Human Myeloperoxidase ELISA Kit Abcam Cat#ab119605 Mouse Myeloperoxidase ELISA Kit Abcam Cat#ab275109 Mouse Direct PCR Kit Selleck B40013 QubitTM dsDNA Quantification Kit Thermo Fisher Cat#Q32851 Reactive Oxygen Species Assay Kit Servicebio Cat#G1706-100T PrimeScript RT reagent kit TaKaRa Cat#RR037A (Continued on next page) e2 Cell Reports Medicine 6, 102140, May 20, 2025 ..

    Isolation:

    Article Title: Targeting mitochondrial complex I of CD177 + neutrophils alleviates lung ischemia-reperfusion injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human CD177 Antibody Biolegend Cat#315806; RRID:AB_2564281 PE/Cyanine7 anti-human CD45 Antibody Biolegend Cat#368532; RRID:AB_2715892 FITC anti-human CD66b Antibody Biolegend Cat#305104; RRID:AB_314496 FITC anti-mouse Ly-6G Antibody Biolegend Cat#127606; RRID:AB_1236494 PE/Cyanine7 anti-mouse/human CD11b Antibody Biolegend Cat#101216; RRID:AB_312799 PerCP-Cyanine5.5 anti-mouse/human CD45 Antibody ThermoFisher Cat#45-0451-82; RRID:AB_1107002 Rabbit polyclonal anti-Histone H3 (citrulline R2 + R8 + R17) Abcam Cat#ab281584 Rat monoclonal anti-Ly-6G BD biosciences Cat#551459; RRID:AB_394206 Mouse monoclonal anti-Histone H1 Abcam Cat#ab218417; RRID:AB_218417 Rabbit monoclonal anti-CD177 Santa Cruz Biotechnology Cat# sc-376329; RRID:AB_10988032 Rabbit polyclonal anti-CD177 Abclonal Cat#A6550; RRID:AB_2767144 Rat monoclonal anti-CD11b Abcam Cat#ab8878; RRID:AB_306831 Rabbit monoclonal anti-CD11b Abcam Cat# ab52478; RRID:AB_868788 Rabbit monoclonal anti-CD11b Abcam Cat#ab184308; RRID:AB_2889154 Mouse monoclonal anti-ILK Protientech Cat#67724-1-Ig; RRID:AB_2882910 Rabbit monoclonal anti-ILK Abcam Cat#ab52480; RRID:AB_881194 Rabbit monoclonal anti-CD11b Abcam Cat#ab133357; RRID:AB_2650514 Rabbit monoclonal anti-AKT Abcam Cat#ab179463; RRID:AB_2810977 Rabbit monoclonal anti-phospho-AKT Abcam Cat#ab192623; RRID:AB_2934087 Rabbit monoclonal anti-mTOR Abcam Cat#ab134903; RRID:AB_2800465 Rabbit monoclonal anti-phospho-mTOR Abcam Cat#ab109268; RRID:AB_10888105 Rabbit polyclonal anti- ATP5A1 Protientech Cat#14676-1-AP; RRID:AB_2061761 Rabbit polyclonal anti-UQCRC2 Protientech Cat#14742-1-AP; RRID:AB_2241442 (Continued on next page) Cell Reports Medicine 6, 102140, May 20, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-SDHA Protientech Cat#14865-1-AP; RRID:AB_11182164 Rabbit polyclonal anti-NDUFS1 Protientech Cat#12444-1-AP; RRID:AB_2282657 Rabbit polyclonal anti-COXIV Protientech Cat#11242-1-AP; RRID:AB_2085278 Rabbit polyclonal anti-GAPDH Servicebio Cat# GB11002-100; RRID:AB_2904017 Rabbit polyclonal anti-β-actin Biolegend Cat#622102; RRID:AB_315946 Biological samples Peripheral blood Shanghai Pulmonary Hospital N/A Chemicals, peptides, and recombinant proteins IACS-010759 MCE Cat#HY-112037 MHY1485 MCE Cat#HY-B0795 AZD8055 MCE Cat#HY-10422 SC-79 MCE Cat#HY-18749 Ipatasertib MCE Cat#HY-15186 QLT0267 Cayman Chemical Cat#866409-68-9 Celsior solution Genzyme Corp NA PolymorphPrep Axis Shield Cat#1114683 Erythrocytes lysate Biosharp Cat#BL503B Human BD Fc Block BD biosciences Cat#564220 Mouse BD Fc Block BD biosciences Cat#553141 FACS buffer BD biosciences Cat#554656 LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit ThermoFisher Cat# L34973 TRIzol Invitrogen Cat#15596018CN Sytox green Alphabio Cat#AP6352 RIPA buffer Beyotime Cat#P0013C PMSF and phosphatase inhibitors Beyotime Cat#P1045 Digitonin Invitrogen Cat#BN2008 Coomassie blue G250 Invitrogen Cat#BN2008 Dark blue cathode running buffer Invitrogen Cat#BN2002 Bright blue cathode running buffer Invitrogen CatBN2007 Electron microscope fixative Servicebio Cat#G1102 MitoSOXTM Deep Red Dojindo Cat#MT14 MitoTrackerTM Red CMXRos Beyotime Cat#C1049B MACS Tissue Storage Solution Miltenyi Cat#130-100-008 Collagenase Type I Thermo Fisher Cat#17100017 Dispase Roche Cat#04942086001 DNase Roche Cat#10104159001 Critical commercial assays Mouse Bone Marrow Neutrophil Isolation Kit Solarbio Cat#P8550 Human Myeloperoxidase ELISA Kit Abcam Cat#ab119605 Mouse Myeloperoxidase ELISA Kit Abcam Cat#ab275109 Mouse Direct PCR Kit Selleck B40013 QubitTM dsDNA Quantification Kit Thermo Fisher Cat#Q32851 Reactive Oxygen Species Assay Kit Servicebio Cat#G1706-100T PrimeScript RT reagent kit TaKaRa Cat#RR037A (Continued on next page) e2 Cell Reports Medicine 6, 102140, May 20, 2025 ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Targeting mitochondrial complex I of CD177 + neutrophils alleviates lung ischemia-reperfusion injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human CD177 Antibody Biolegend Cat#315806; RRID:AB_2564281 PE/Cyanine7 anti-human CD45 Antibody Biolegend Cat#368532; RRID:AB_2715892 FITC anti-human CD66b Antibody Biolegend Cat#305104; RRID:AB_314496 FITC anti-mouse Ly-6G Antibody Biolegend Cat#127606; RRID:AB_1236494 PE/Cyanine7 anti-mouse/human CD11b Antibody Biolegend Cat#101216; RRID:AB_312799 PerCP-Cyanine5.5 anti-mouse/human CD45 Antibody ThermoFisher Cat#45-0451-82; RRID:AB_1107002 Rabbit polyclonal anti-Histone H3 (citrulline R2 + R8 + R17) Abcam Cat#ab281584 Rat monoclonal anti-Ly-6G BD biosciences Cat#551459; RRID:AB_394206 Mouse monoclonal anti-Histone H1 Abcam Cat#ab218417; RRID:AB_218417 Rabbit monoclonal anti-CD177 Santa Cruz Biotechnology Cat# sc-376329; RRID:AB_10988032 Rabbit polyclonal anti-CD177 Abclonal Cat#A6550; RRID:AB_2767144 Rat monoclonal anti-CD11b Abcam Cat#ab8878; RRID:AB_306831 Rabbit monoclonal anti-CD11b Abcam Cat# ab52478; RRID:AB_868788 Rabbit monoclonal anti-CD11b Abcam Cat#ab184308; RRID:AB_2889154 Mouse monoclonal anti-ILK Protientech Cat#67724-1-Ig; RRID:AB_2882910 Rabbit monoclonal anti-ILK Abcam Cat#ab52480; RRID:AB_881194 Rabbit monoclonal anti-CD11b Abcam Cat#ab133357; RRID:AB_2650514 Rabbit monoclonal anti-AKT Abcam Cat#ab179463; RRID:AB_2810977 Rabbit monoclonal anti-phospho-AKT Abcam Cat#ab192623; RRID:AB_2934087 Rabbit monoclonal anti-mTOR Abcam Cat#ab134903; RRID:AB_2800465 Rabbit monoclonal anti-phospho-mTOR Abcam Cat#ab109268; RRID:AB_10888105 Rabbit polyclonal anti- ATP5A1 Protientech Cat#14676-1-AP; RRID:AB_2061761 Rabbit polyclonal anti-UQCRC2 Protientech Cat#14742-1-AP; RRID:AB_2241442 (Continued on next page) Cell Reports Medicine 6, 102140, May 20, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-SDHA Protientech Cat#14865-1-AP; RRID:AB_11182164 Rabbit polyclonal anti-NDUFS1 Protientech Cat#12444-1-AP; RRID:AB_2282657 Rabbit polyclonal anti-COXIV Protientech Cat#11242-1-AP; RRID:AB_2085278 Rabbit polyclonal anti-GAPDH Servicebio Cat# GB11002-100; RRID:AB_2904017 Rabbit polyclonal anti-β-actin Biolegend Cat#622102; RRID:AB_315946 Biological samples Peripheral blood Shanghai Pulmonary Hospital N/A Chemicals, peptides, and recombinant proteins IACS-010759 MCE Cat#HY-112037 MHY1485 MCE Cat#HY-B0795 AZD8055 MCE Cat#HY-10422 SC-79 MCE Cat#HY-18749 Ipatasertib MCE Cat#HY-15186 QLT0267 Cayman Chemical Cat#866409-68-9 Celsior solution Genzyme Corp NA PolymorphPrep Axis Shield Cat#1114683 Erythrocytes lysate Biosharp Cat#BL503B Human BD Fc Block BD biosciences Cat#564220 Mouse BD Fc Block BD biosciences Cat#553141 FACS buffer BD biosciences Cat#554656 LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit ThermoFisher Cat# L34973 TRIzol Invitrogen Cat#15596018CN Sytox green Alphabio Cat#AP6352 RIPA buffer Beyotime Cat#P0013C PMSF and phosphatase inhibitors Beyotime Cat#P1045 Digitonin Invitrogen Cat#BN2008 Coomassie blue G250 Invitrogen Cat#BN2008 Dark blue cathode running buffer Invitrogen Cat#BN2002 Bright blue cathode running buffer Invitrogen CatBN2007 Electron microscope fixative Servicebio Cat#G1102 MitoSOXTM Deep Red Dojindo Cat#MT14 MitoTrackerTM Red CMXRos Beyotime Cat#C1049B MACS Tissue Storage Solution Miltenyi Cat#130-100-008 Collagenase Type I Thermo Fisher Cat#17100017 Dispase Roche Cat#04942086001 DNase Roche Cat#10104159001 Critical commercial assays Mouse Bone Marrow Neutrophil Isolation Kit Solarbio Cat#P8550 Human Myeloperoxidase ELISA Kit Abcam Cat#ab119605 Mouse Myeloperoxidase ELISA Kit Abcam Cat#ab275109 Mouse Direct PCR Kit Selleck B40013 QubitTM dsDNA Quantification Kit Thermo Fisher Cat#Q32851 Reactive Oxygen Species Assay Kit Servicebio Cat#G1706-100T PrimeScript RT reagent kit TaKaRa Cat#RR037A (Continued on next page) e2 Cell Reports Medicine 6, 102140, May 20, 2025 ..

    Polymerase Chain Reaction:

    Article Title: Targeting mitochondrial complex I of CD177 + neutrophils alleviates lung ischemia-reperfusion injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-human CD177 Antibody Biolegend Cat#315806; RRID:AB_2564281 PE/Cyanine7 anti-human CD45 Antibody Biolegend Cat#368532; RRID:AB_2715892 FITC anti-human CD66b Antibody Biolegend Cat#305104; RRID:AB_314496 FITC anti-mouse Ly-6G Antibody Biolegend Cat#127606; RRID:AB_1236494 PE/Cyanine7 anti-mouse/human CD11b Antibody Biolegend Cat#101216; RRID:AB_312799 PerCP-Cyanine5.5 anti-mouse/human CD45 Antibody ThermoFisher Cat#45-0451-82; RRID:AB_1107002 Rabbit polyclonal anti-Histone H3 (citrulline R2 + R8 + R17) Abcam Cat#ab281584 Rat monoclonal anti-Ly-6G BD biosciences Cat#551459; RRID:AB_394206 Mouse monoclonal anti-Histone H1 Abcam Cat#ab218417; RRID:AB_218417 Rabbit monoclonal anti-CD177 Santa Cruz Biotechnology Cat# sc-376329; RRID:AB_10988032 Rabbit polyclonal anti-CD177 Abclonal Cat#A6550; RRID:AB_2767144 Rat monoclonal anti-CD11b Abcam Cat#ab8878; RRID:AB_306831 Rabbit monoclonal anti-CD11b Abcam Cat# ab52478; RRID:AB_868788 Rabbit monoclonal anti-CD11b Abcam Cat#ab184308; RRID:AB_2889154 Mouse monoclonal anti-ILK Protientech Cat#67724-1-Ig; RRID:AB_2882910 Rabbit monoclonal anti-ILK Abcam Cat#ab52480; RRID:AB_881194 Rabbit monoclonal anti-CD11b Abcam Cat#ab133357; RRID:AB_2650514 Rabbit monoclonal anti-AKT Abcam Cat#ab179463; RRID:AB_2810977 Rabbit monoclonal anti-phospho-AKT Abcam Cat#ab192623; RRID:AB_2934087 Rabbit monoclonal anti-mTOR Abcam Cat#ab134903; RRID:AB_2800465 Rabbit monoclonal anti-phospho-mTOR Abcam Cat#ab109268; RRID:AB_10888105 Rabbit polyclonal anti- ATP5A1 Protientech Cat#14676-1-AP; RRID:AB_2061761 Rabbit polyclonal anti-UQCRC2 Protientech Cat#14742-1-AP; RRID:AB_2241442 (Continued on next page) Cell Reports Medicine 6, 102140, May 20, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit polyclonal anti-SDHA Protientech Cat#14865-1-AP; RRID:AB_11182164 Rabbit polyclonal anti-NDUFS1 Protientech Cat#12444-1-AP; RRID:AB_2282657 Rabbit polyclonal anti-COXIV Protientech Cat#11242-1-AP; RRID:AB_2085278 Rabbit polyclonal anti-GAPDH Servicebio Cat# GB11002-100; RRID:AB_2904017 Rabbit polyclonal anti-β-actin Biolegend Cat#622102; RRID:AB_315946 Biological samples Peripheral blood Shanghai Pulmonary Hospital N/A Chemicals, peptides, and recombinant proteins IACS-010759 MCE Cat#HY-112037 MHY1485 MCE Cat#HY-B0795 AZD8055 MCE Cat#HY-10422 SC-79 MCE Cat#HY-18749 Ipatasertib MCE Cat#HY-15186 QLT0267 Cayman Chemical Cat#866409-68-9 Celsior solution Genzyme Corp NA PolymorphPrep Axis Shield Cat#1114683 Erythrocytes lysate Biosharp Cat#BL503B Human BD Fc Block BD biosciences Cat#564220 Mouse BD Fc Block BD biosciences Cat#553141 FACS buffer BD biosciences Cat#554656 LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit ThermoFisher Cat# L34973 TRIzol Invitrogen Cat#15596018CN Sytox green Alphabio Cat#AP6352 RIPA buffer Beyotime Cat#P0013C PMSF and phosphatase inhibitors Beyotime Cat#P1045 Digitonin Invitrogen Cat#BN2008 Coomassie blue G250 Invitrogen Cat#BN2008 Dark blue cathode running buffer Invitrogen Cat#BN2002 Bright blue cathode running buffer Invitrogen CatBN2007 Electron microscope fixative Servicebio Cat#G1102 MitoSOXTM Deep Red Dojindo Cat#MT14 MitoTrackerTM Red CMXRos Beyotime Cat#C1049B MACS Tissue Storage Solution Miltenyi Cat#130-100-008 Collagenase Type I Thermo Fisher Cat#17100017 Dispase Roche Cat#04942086001 DNase Roche Cat#10104159001 Critical commercial assays Mouse Bone Marrow Neutrophil Isolation Kit Solarbio Cat#P8550 Human Myeloperoxidase ELISA Kit Abcam Cat#ab119605 Mouse Myeloperoxidase ELISA Kit Abcam Cat#ab275109 Mouse Direct PCR Kit Selleck B40013 QubitTM dsDNA Quantification Kit Thermo Fisher Cat#Q32851 Reactive Oxygen Species Assay Kit Servicebio Cat#G1706-100T PrimeScript RT reagent kit TaKaRa Cat#RR037A (Continued on next page) e2 Cell Reports Medicine 6, 102140, May 20, 2025 ..



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